Jyrki Heino - Selected Publications#

A.M. Salo, P. Rappu, M.K. Koski, E. Karjalainen, V. Izzi, K. Drushinin, I. Miinalainen, J. Käpylä, J. Heino and J. Myllyharju (2024) Collagen prolyl 4-hydroxylase isoenzymes I and II have sequence specificity towards different X-Pro-Gly triplets. Matrix Biology 125: 73-87. -

This paper solves the long-standing question about the substrate specificity of collagen modifying enzymes.

E. Siljamäki, P. Riihilä, U. Suwal, L. Nissinen, P. Rappu, M. Kallajoki, V-M. Kähäri and J. Heino (2023) Inhibition of TGF-β signaling, invasion and growth of cutaneous squamous cell carcinoma by PLX8394. Oncogene 42: 3633-3647.

This report indicates that PLX8394 (a candidate melanoma drug) is a new promising candidate to be used in the treatment metastatic human cutaneous squamous cell carcinoma, based on previously unknown properties of PLX8394.

J. Heino and E. Siljamäki (2023) Integrins alpha1beta1 and alpha2beta1 – The generalist collagen receptors. In Integrins in Health and Disease: Key Effectors of Cell-Matrix and Cell-Cell Interactions (Editors: D. Gullberg, J. Eble). The Biology of Extracellular Matrix 13, Springer, pp. 3-25.

A well-received (see: Current Opinion in Cell Biology, 2023, 85:102273) summary of collagen receptor integrins, including the contributions of Heino lab.

K. Sipilä, E. Rognoni, J. Jokinen, M. Tewary, M. Vietri-Rudan, S. Talvi, V. Jokinen, K.M. Dahlström, K. Liakath-Ali, X. Du, J. Käpylä, S. Nutt, T. Salminen, J. Heino and F.M. Watt (2022) Embigin is a novel fibronectin receptor that regulates sebaceous gland differentiation. Developmental Cell 57: 1453-1465.

A novel fibronectin receptor found in Heino's research group. It's role in sebocytes unveiled in collaboration with Professor Fiona Watt's group.

A. Peirsman, E. Blondeel, T. Ahmed, J. Anckaert, D. Audenaert, T. Boterberg, K. Buzas, N. Carragher, G. Castellani, F. Castro, V. Dangles-Marie, J. Dawson, P. De Tullio, E. De Vlieghere, S. Dedeyne, H. Depypere, A. Diosdi, R.I. Dmitriev, H. Dolznig, S. Fischer, C. Gespach, V. Goossens, J. Heino, A. Hendrix, Peter Horvath, L.A. Kunz-Schughart, S. Maes, C. Mangodt, P. Mestdagh, S. Michlikova, M.J. Oliveira, F. Pampaloni, F. Piccinini, C. Pinheiro, J. Rahn, S.M. Robbins, E. Siljamäki, P. Steigemann, G. Sys, S. Takayama, A. Tesei, J. Tulkens, M. Van Waeyenberge, J. Vandesompele, G. Wagemans, C. Weindorfer, N. Yigit, N. Zablowsky, M. Zanoni, P. Blondeel, and O. De Wever (2021) MISpheroID: a knowledgebase and transparency tool for Minimal Information in Spheroid IDentity. Nature Methods 18: 1294-1303.

Establishment of a large international consortium aiming to standardize 3D cell culture methods and to promote repeatable research and open science.promote repeatable research and open science.

E. Siljamäki, P. Rappu, P. Riihilä, L. Nissinen, V-M. Kähäri, and J. Heino (2020) H-Ras activation and fibroblast-induced TGF-β signaling promote laminin-332 accumulation and invasion in cutaneous squamous cell carcinoma. Matrix Biology 87: 26-47.

Molecular mechanism of invasion by Ras-activated skin cancer cells.

P. Kankaanpää, L. Paavolainen, S. Tiitta, M. Karjalainen, J. Päivärinne, J. Nieminen, V. Marjomäki, J. Heino, and D.J. White (2012) BioImageXD: an open, general-purpose and high-throughput image-processing platform. Nature Methods 9:683-689.

A novel image-processing platform.

L. Nissinen, J. Koivunen, J. Käpylä, M. Salmela, J. Nieminen, J. Jokinen, K. Sipilä, M. Pihlavisto, O.T. Pentikäinen, A. Marjamäki, and J. Heino (2012) Novel alpha2beta1 integrin inhibitors reveal that integrin binding to collagen under shear stress conditions does not require receptor preactivation. J Biol Chem 287:44694-44702.

Description of novel small molecular inhibitors for different conformations of alpha2 integrin. One of the inhibitors (BTT-3033) has been made widely available by many commercial distributors of scientific reagents and recently it has been used by numerous research groups in their published papers.

H. Larjava, T. Salo, K. Haapasalmi, R. Kramer, and J. Heino (1993) Expression of integrins and basement membrane components by wound keratinocytes. J Clin Invest 92: 1425-1435.

First comprehensive description of major integrins and matrix proteins during human mucosal wound healing. Cited 331 times (Web of Science), 521 times (Google Scholar).

J. Heino, R.A. Ignotz, M.E. Hemler, C. Crouse, and J. Massagué (1989) Regulation of cell adhesion receptors by TGF-beta. Concomitant regulation of integrins that share a common beta1-subunit. J Biol Chem 264: 380-388.

Landmark paper describing the basics of beta1-integrin heterodimer formation. Indication that beta1-integrins cannot exist as trimers (as had been proposed). Cited 642 times (Web of Science), 820 times (Google Scholar).